IR Spectroscopy is based on the vibrations of atoms in a molecule, and the idea behind the IR spectrum is by passing the infrared radiation in to a target sample and obtaining the absorption spectra at a particular energy. these changes in the interaction of energy with matter is because of the freedom of rotational and vibrational physics in an atom[1].
Infrared spectroscopy has wide range of applications in blood serum. the proteins, lipids,carbohydrates etc of red blood cells have a sense of absorption spectra in Infrared region due to the nature of the bond between carbon-oxygen, carbon-nitrogen and nitrogen-oxygen etc. so many medical applications like heart surgeries, coronary treatments use this instrument for anaysis [1].
References:
[1] A book called "Biological Applications of Infrared Spectrscopy" by Barbara Staurt, University of Technology, Sydney, Australia.
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